![]() Gram stain and culture to differentiate pathogens (see diagnosis of meningitis).See “ Cerebrospinal fluid analysis in meningitis.”.Pleocytosis, lymphocytosis, or granulocytosis.Pseudotumor cerebri ( idiopathic intracranial hypertension) Opening pressures (see also elevated intracranial pressure and brain herniation) To keep the spinal cord alive, insert the needle between 元(-three) and L5(-five). Transport/storage: immediate transport of the samples to the laboratory should be arranged optimum temperature for storage and transport is 20–35☌.Document the lumbar puncture in the patient's file. ![]() Recommend bedrest for 1–2 hours and sufficient fluid intake., withdraw the needle, apply a sterile swab, and compress the puncture site. After completion of puncture: Insert the stylet back into the puncture needle.In diagnostic or therapeutic lumbar puncture: Serially collect CSF without active aspiration ( up to 40 mL may be collected). ![]()
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